Institute of Pathology, Charité

Universitätsmedizin Berlin

E-Mail: mihnea.dragomir@charite.de

Project Title: Using DNA methylation profiling for differential diagnosis and for determining the origin of ovarian cancers

My research interests lie in classifying, solving differential diagnosis problems, and determining the origin of ovarian cancers. We are using DNA methylation data to develop machine learning algorithms for analyzing ovarian tumors. DNA methylation patterns are highly tissue specific, hence are an ideal method for performing differential diagnosis. Currently, we aim to develop a machine learning classifier that can differentiate subtypes of ovarian carcinomas. Additionally, we aim to use DNA methylation data to predict the outcome of and to subclassify ovarian carcinomas. We plan to expand our research to other female reproductive system tumors.

Department of Radiation Oncology, Charité - Universitätsmedizin Berlin

Email: felix.ehret@charite.de

Project title: The Role of Radiotherapy in the Treatment of Atypical Meningiomas – A Methylome- and Genome-based Analysis.

The role of an adjuvant radiotherapy for grade 2 meningiomas remains unclear. The traditional approach of meningioma diagnosis using only light microscopy and immunohistochemistry to distinguish the current subclasses is prone to interobserver biases and limited regarding personalized treatment decision making. This knowledge gap is highlighted by contradictory findings in various studies. By implementing a DNA-methylation-based classifier and incorporating genetic risk factors to ensure accurate tumor subtype identification, the project aims to specifically assess the role of adjuvant radiotherapy in a homogenous meningioma cohort.

Functional genomics of paediatric cancers group, Experimental and Clinical Research Center (ECRC) of the Max Delbrück Center (MDC) and Charité Berlin, Germany

Postdoctoral Computational Biologist

E-Mail: kerstin.haase@charite.de

Project Title: Functional relevance of DNA circularization and its contribution to genetic heterogeneity and evolution in childhood solid tumours

My work aims to assess changes in extrachromosomal circular DNA (ecDNA) heterogeneity over time using various cell types and sequencing techniques. Insights from these data will help determine how circular DNA elements are selected for and expressed in the context of enhanced proliferation. Understanding the nature and clinical importance of ecDNA is currently limited by our ability to reconstruct entire circles and genomic fragments contained therein. Hence, my project involves adaption and integration of existing methods, as well as development of new algorithms to reliably quantify and reconstruct ecDNA elements from bulk and single-cell sequencing data at clinically relevant time points.

Charité – Universitätsmedizin Berlin

Medizinische Klinik mit Schwerpunkt Hämatologie, Onkologie und Tumorimmunologie (CBF)

Hindenburgdamm 30
12203 Berlin

E-Mail: stefan.habringer@charite.de 

Project Title: Identification and Targeting of Novel Target Structures in B Cell Lymphoma

B cell lymphomas (BCL) are genetically heterogeneous diseases, yet standard treatment is still primarily based on chemotherapy and CD20-antibodies, irrespective of molecular alterations. Therefore, targeted treatment strategies exploiting specific vulnerabilities of molecular subtypes are urgently needed. By means of forward-genetic, genome-wide screening using the PiggyBac transposon-transposase system in BCL-prone mouse models we are working on i) the identification of novel oncogenes and tumor suppressors, ii) deciphering mechanisms of lymphomagenesis and therapy resistance and iii) translation of our findings into molecularly-informed, targeted treatment strategies.

(© Charité – Universitätsmedizin Berlin)

Charité Universitaetsmedizin Berlin

Department Dermatology, Venereology and Allergology

AG Molecular Immunology Charité
Head of Experimental Research

Charitéplatz 1
10117 Berlin

E-Mail: franz.hilke@charite.de

Project Title: Identification and validation of genetic and cellular biomarkers for patient stratification, prognosis and early detection of resistance mechanisms in the context of tumor therapy.

My research interests lie in understanding and deciphering the molecular mechanisms underlying tumorigenesis and cause targeted and/or immune checkpoint inhibitor therapies to fail. Currently, we focus on biobanking for the purpose of comprehensive genomic tumor characterization and the implementation of liquid biopsy. Second, we study immune adverse events, especially in the skin, during immune checkpoint therapy to understand and compare the underlying molecular mechanism to inflammatory and autoimmune diseases of the skin. Third, we are interested in the different compartments of the immune system fighting cancer, particularly the role of the dendritic cells and the killing by granzymB.

Department of Hematology and Oncology

Charité – Universitätsmedizin Berlin

Berlin

E-Mail: arunima.murgai@charite.de

Protein ubiquitination is a post-translational modification that leads to proteasomal degradation or altered function of a protein. My project aims to investigate the oncogenic role of deubuquitinating enzymes (DUBs) and their exploitation as drug targets in Acute Myeloid Leukemia (AML). Our preliminary CRISPR screenings for DUB inactivation identified USP7 to be essential for several AML cell lines. A combinatorial approach encompassing proteomic, biochemistry and molecular biology analyses will aim to identify novel the substrates of USP7 which are critical for AML. The results of this project will help to further understand the role of the ubiquitin-proteasome system in the pathogenesis of AML and to identify new therapeutic approaches.

Department for Hematology, Oncology and Tumor Immunology

German Cancer Consortium (DKTK), partner site Berlin

Campus Virchow Clinic, Charité Berlin

E-Mail: daniel.noerenberg@charite.de

Project Title: Genetic Characterization of Primary Mediastinal B cell Lymphoma

Unravelling genetic aberrations underlying PMBL lymphomagenesis has the potential to identify new targets for tailored therapy approaches. A thorough description of the mutational spectrum in PMBL and the identification of key oncogenic drivers will thus facilitate rational therapeutic approaches. Until now, we have collected the world’s largest PMBL cohort (n>400) through national and international collaborations comprising clinically well annotated patients. Using a combination of whole-exome, targeted deep resequencing and gene expression analysis, we aim to identify key oncogenic drivers and deregulated signaling pathways in PMBL. Based on the previous molecular analyses, functional consequences of candidate driver mutations will be analyzed in PMBL cell lines using the CRISPR/Cas technology.

(© Charité – Universitätsmedizin Berlin)

Deutsches Konsortium für Translationale Krebsforschung (DKTK)

Deutsches Krebsforschungszentrum (DKFZ)

E-Mail: anacristina.afonsecapestana@dkfz-heidelberg.de

Charité –Universitätsmedizin Berlin

Klinik für Radioonkologie und Strahlentherapie

Labor für Strahlenbiologie

Charitéplatz 1, Hufelandweg 9
10117 Berlin

E-Mail: ana.pestana@charite.de​

Project Title: Tracking therapy-driven clonal evolution to understand treatment resistance in head neck cancer

​The project has the objective to understand Head and Neck Cancers clonal heterogeneity and its role in resistance to treatment. We will use primary cells derived from patient’s samples or PDX models that will be cultured in 3D conditions (organoids). Through RGB and/or molecular barcoding, each clone composing the tumour bulk will be marked and therefore distinguishable from the others. Taking advantage of this, we will be able to identify the resistant clones and their expression profiles pinpointing what confers them resistance. We believe that this work will allow us to identify possible therapeutic targets for each treatment, and in this way contribute to improve HNSCC treatment options.

Department of Nuclear Medicine

Charité – Universitätsmedizin Berlin

Augustenburger Platz 1

D-13353 Berlin, Germany

E-Mail: julian.rogasch@charite.de

Project Title: Image-derived biomarkers from FDG-PET/CT for staging and prognosis in non-small cell lung cancer

The research aims at improving non-invasive pretherapeutic staging using FDG-PET/CT in patients with non-small cell lung cancer (NSCLC) using image-derived biomarkers (radiomics) and machine learning-based classifiers. A special focus is to improve thoracic lymph node staging. Furthermore, biomarkers are used to develop more differentiated prognostic models to predict patient survival after curatively intended treatment.

(© Charité – Universitätsmedizin Berlin)

Department of Hematology, Oncology, and Tumor Immunology

Charité - Universitätsmedizin Berlin

Augustenburger Platz 1

D-13353 Berlin, Germany

Humboldt Postdoctoral Research Fellow

Email: paulina.strzelecka@charite.de

Project Title: Clonal haematopoiesis of indeterminate potential (CHIP) at single-cell resolution

Clonal haematopoiesis of indeterminate potential (CHIP) is an age-related condition characterized by the presence of somatic mutation(s) in a haematologic cancer-associated gene in a blood of otherwise healthy individuals. In affected individuals, a substantial proportion of mature blood cells is derived from a dominant stem cell that acquired the mutation. My project focuses on the application of single-cell techniques (single-cell transcriptomics and single-cell epigenetics) to decipher the changes in a population of blood cells stemming from the presence of the mutations. The results are expected to reveal how somatic mutations affect cellular programmes in a cell type specific manner. 

Department of Radiation Oncology

Charité – Universitätsmedizin Berlin

 Augustenburger Platz 1, 13353 Berlin

Email: siyer.roohani@charite.de    

Link institutional website
Link Sarkom-Zentrum Charité website

Project title: Breaking Radioresistance of Soft Tissue Sarcomas by New Combination Therapies: Development of a Preclinical Pipeline

Despite their radioresistance and heterogeneity of over 70 subtypes, all soft tissue sarcomas (STS) are treated with the same perioperative radiotherapy regimen. Preclinical and clinical data on effective combination therapies of radiation and targeted compounds is scarce. This proof-of-concept study uses preclinical 3D models of STS to test two hypotheses: 1) Differences in radioresistance among STS subtypes can be reproducibly shown in 3D STS models; 2) New combination therapies of radiation + targeted compounds can overcome radioresistance in STS and be demonstrated in 3D STS models. The study lays the foundation for a preclinical drug and radiation screening platform for STS.
 

Experimental Neurosurgery/Tumor immunology

Department of Neurosurgery

University Hospital Carl Gustav Carus

Dresden, Germany

Group leader “NK cell-based immunotherapies of malignant neoplasias”

E-Mail: susanne.michen@ukdd.de

Project Title: Development of novel immunotherapies with genetically modified primary NK cells for cancer treatment

We are focusing on NK cell-based immunotherapy of solid tumors, especially brain tumors such as glioblastoma multiforme. NK cells are able to recognize and kill malignant cells with loss of HLA class I expression and concomitant upregulation of stress ligands for activating NK cell receptors. However, most glioma cells express HLA class I molecules and NK cells are additionally blocked by immune-suppressive HLA-E and HLA-G molecules. To overcome this, we generate CAR-modified NK cells directed against various surface-exposed tumor-associated antigens. However, the expansion of these CAR-NK cells to clinically applicable numbers still represents a major hurdle. Therefore, our research focuses on novel methods for selective expansion of CAR-NK cells.

Institut für Zelltherapeutische Forschung

Universitätsklinikum Essen, Deutschland

www.uk-essen.de/en/zelltherapeutische-forschung/

E-Mail: esteban.arrieta-bolanos@uk-essen.de

Project Title: Harnessing the biological principles of alloreactivity for immunotherapy of hematological malignancies

We are interested in understanding the biological principles of T-cell alloreactivity, in particular against the HLA class II molecule HLA-DP, in the context of hematopoietic cell transplantation (HCT) for the treatment of hematological malignancies. Our research focuses on unraveling determinants of harnessed alloreactive responses, looking at both sides of the immunological equation, i.e. HLA immunopeptidomics and alloreactive T-cell diversity. Our aim is to identify therapeutic approaches and interventions, as well as molecular and cellular products with translational application, in order to manipulate alloreactive responses and improve leukemia immunotherapy and the efficacy of HCT.

Department of Human Genetics & Department of Pediatric Hematology and Oncology University Hospital Essen, University of Duisburg-Essen

E-Mail: Nicole.Barwinski@uk-essen.de

Project Title: cf-DNA and EVs as sources for biomarkers for early detection of second primary malignancies in patients with heritable retinoblastoma

Heritable retinoblastoma is a tumour predisposition syndrome caused by heterozygosity for pathogenic RB1 variants. Patients have a high risk to develop a second primary malignancy later in life. Development of such tumours is often initiated by loss of heterozygosity of the RB1 gene. The aim of our project is to develop a noninvasive blood test for the early detection of these secondary cancers. Therefore we investigate cfDNA released by tumour cells to detect the allelic imbalance at the RB1 gene locus. Using the SimSenSeq technology (Ståhlberg et al. 2017) we perform Next-Generation Sequencing to determine the allelic ratio with high sensitivity and specificity to reach the requirements of a diagnostic test.

DKFZ-Division Translational Neurooncology at the West German Cancer Center (WTZ)

DKTK partner site Essen/Düsseldorf

Universitätsklinikum Essen

Hufelandstrasse 55

45147 Essen

E-Mail: c.dobersalske@dkfz.de

Project Title: Tumor microenvironment and resistance mechanisms in malignant glioma

The dynamic and heterogenous cell composition in the tumor microenvironment (TME) actively influences therapeutic efficacy in all different kinds of tumor entities. Our aim is to unravel the insufficiently characterized TME in glioma by establishing models that are acutely maintain the TME of clinical specimen. We firmly believe that understanding and further targeting microenvironmental factors is indispensable to allow the identification of appropriate treatment strategies and to potentially overcome therapy resistance.

Institute of Cell Biology (Cancer Research)

University Hospital Essen, University of Duisburg-Essen

45147 Essen, Germany

E-Mail: johann.matschke@uk-essen.de

Project Title: Role of Metabolism in Radiation response

Our research focuses on the role of metabolic reprogramming in cancer cells for intrinsic, microenvironment-induced, and adaptive radioresistance and the identification of associated specific metabolic vulnerabilities of cancer cells suited to improve the cytotoxic efficacy of radiotherapy.

Therefore, we have established various specific in vitro methods for the analysis and evaluation of radiobiology endpoints, cell metabolite levels, real-time metabolic fluxes in normoxia and hypoxia (Seahorse Bioanalyser). We also perform time-resolved metabolome analyses with national and inter-national cooperation partners. Long-term goal is to develop effective strategies for a targeted cancer cell radiosensitization and to identify associated stratification markers.

Klinik für Neurochirurgie und Wirbelsäulenchirurgie der Universitätsmedizin Essen 

Deutsches Krebsforschungszentrum – Abteilung Translationale Neuroonkologie am 

Westdeutschen Tumorzentrum (WTZ) der Universitätsmedizin Essen

E-Mail: laurel.rauschenbach@uk-essen.de

Project Title: Identification and validation of targeting approaches in primary and secondary brain and spinal cord malignancies

Our research activities focus on understanding and development of diagnostic and therapeutic approaches in central nervous system malignancies, with special emphasis on stem cell technologies, microenvironment studies and immunotherapy in glioma, metastases and leptomeningeal cancer spread. To accomplish this, we are investigating patient-derived tumor cells and xenograft models of primary and metastasized brain or spinal cord tumors. For biomarker and target discovery approaches, our experimental findings are translated to early clinical trials. 

Department of Hematology/Medical Oncology

University Hospital Frankfurt, Germany

E-Mail: julius.enssle@kgu.de

Project Title: Functional proteogenomic characterization of mantle cell lymphoma

Our research aims at expanding the characterization of mantle cell lymphoma (MCL) by mass spectrometry quantitative proteome profiling of primary MCL samples together with whole-exome sequencing, transcriptome sequencing data and clinical outcome data to obtain a multilevel proteogenomic dataset. By bioinformatic integration, we aim at identifying proteogenomic clusters with prognostic relevance and gain insight into the underlying biologic processes. Additionally, analysis of genetic vulnerabilities will allow functional validation of these findings and evaluation of potential biomarkers and novel therapeutic targets to translate the results into novel clinical trials in MCL.

Department of Otorhinolaryngology

University Hospital Frankfurt/Main

Theodor-Stern-Kai 7; Haus 8D

60590 Frankfurt am Main

E-Mail: christian.issing@kgu.de

Project Title: “Patient-derived tumor organoids from head and neck squamous cell carcinoma as a co-clinical research platform”

Head and neck squamous cell carcinoma (HNSCC) are among the most common malignant neoplasms worldwide. While locally limited disease can be cured by surgery and/or radiochemotherapy, a multimodal and potentially side-effect rich therapy regimen is required for advanced HNSCC. The genetic heterogeneity and distinct aetiologies of HNSCC significantly complicates the therapy and makes an individualized therapeutic approach indispensable for a promising long-term treatment. Patient-derived tumor organoids (PDTOs) could provide a                                                                       preclinincal model to test treatment responses in vitro.

Élément Christian20Issing.jpg image

University Cancer Center (UCT), University Medical Center (UMC) of the Johannes Gutenberg University and German Cancer Consortium (DKTK), Partner Site Frankfurt/Mainz, Germany Center for Thrombosis and Hemostasis (CTH), Institute for Clinical Chemistry and Laboratory Medicine

E-Mail: Essak.Khan@unimedizin-mainz.de

Project Title: Disentangling tumor tissue specific regulatory mechanisms of prothrombin (proF2) gene expression by differential high-content screening in an integrated genetic reporter system

In this project, we are interested in developing a standardized methodology for systematically dissecting the tumor-specific disease entities and mechanism that promote extrahepatic proF2 involved in cancer progression using  a transgenic reporter mouse model (Nourse et al., 2021). In a proof of concept, the suitability of this experimental setup to perceive hitherto unknown sources of detrimental extrahepatic proF2 has been  confirmed (Nourse et al., 2021) which corresponds to findings from tumor patients (Xue et al., 2010). A broad direct in vivo RNAi screening (Wuestefeld et al., 2013) and counter validation in human patient’s samples is expected to unravel novel insights into disease relevant entities and pave the way for developing new therapeutic concepts.

Department of Urology

University Hospital Frankfurt

Frankfurt, Germany

E-Mail: Florestanjohannes.koll@kgu.de

Porject Title: Molecular and histological subtyping of muscle-invasive bladder cancer

Muscle-invasive bladder cancer (MIBC) is a heterogeneous disease with poor oncologic outcome. Six different molecular subtypes of bladder cancer have been identified by transcriptomic profiling. These classes differ regarding underlying oncogenic mechanisms, histology and clinical characteristics, including outcomes. But so far, no clinical translation and application of molecular subtypes has occurred. We aim to validate a robust method to define molecular subtypes in MIBC and to correlate these with histological subtypes, response to chemotherapy and survival-rates.

University Cancer Center (UCT), University Medical Center (UMC) of the Johannes Gutenberg University and German Cancer Consortium (DKTK)

Partner Site Frankfurt/Mainz, Germany Department of Internal Medicine III (Hematology, Oncology, Pneumology)

E-Mail: korbinian.kropp@unimedizin-mainz.de

Project Title: Targeted knock-in of an HLA-independent, TRP2-specific (chimerized) T-cell receptor using CRISPR/Cas9 and homology-directed repair templates.

In this project, we investigate a novel approach to the generation of transgenic T cells based on the genome-editing platform CRISPR/Cas9. Following the methods of Eyquem et al. (Nature 2017, 543:113-117) and Roth et al. (Nature 2018, 559:405-409) we place tumor-reactive TCRs at the endogenous TCR locus thereby knocking out endogenous TCR expression in a one-step knockout/knock-in approach. This enhances expression of transferred TCRs, minimizes mispairing and thus may ultimately improve safety of patients in potential future translational approaches. In particular, we redirect T cells against melanoma by knock-in of an HLA-independent TRP2-specific TCR.

University Cancer Center (UCT), University Medical Center (UMC) of the Johannes Gutenberg University and German Cancer Consortium (DKTK), Partner Site Frankfurt/Mainz, Germany Department of Internal Medicine III (Hematology, Oncology, Pneumology)

E-Mail: viral.shah@uni-mainz.de

Project Title: Enhancing PARP inhibition mediated DNA Damage and leveraging inherent anti-apoptotic dependencies in acute myeloid leukemia

Acute myeloid leukemia (AML) is characterized by increased proliferation, evasion of apoptotic stimuli, and block of differentiation.In this project, we aim at shedding light on mechanisms involved in maintaining genomic stability, exploring pathways regulating DSB repair and exploit mechanisms that prevent induction of apoptosis in highly proliferating AML cells.

Dr. Senckenberg Institute of Neurooncology

University Hospital Frankfurt/Main

E-Mail: pia.zeiner@kgu.de

Project Title: Interaction between glioma-associated microglia/macrophages and tumor cells in the context of targeted cancer therapies and immunotherapies

Short description: Our research is focusing on the investigation of the immunological response in the microenvironment of brain tumors with a special focus on glioma-associated microglia and macrophages (GAMs). In particular, we assess the influence of established and novel targeted therapies on GAM functions and the potential impact on therapeutic response in glioblastoma patients. One aim of the study is the detailed characterization of the mTOR-signalling cascade as a central regulator and potential therapeutic target in GAMs. 

MSNZ Research Fellow I Medical Scientist

Institute of Neuroradiology
Research Group “Translational Oncology”
University Hospital Frankfurt

E-Mail: seyma.alcicek@kgu.de 

​​​​​​​Research Focus: Non-invasive Imaging of Tumor Metabolism Using In vivo Magnetic Resonance Spectroscopy 

Magnetic resonance spectroscopic imaging (MRSI) is a non-invasive technique that enables in vivo observation of tissue metabolism by exploiting the magnetic properties of cell metabolites. My research focuses on improving this methodology and its postprocessing methods to monitor altered cellular metabolism, the hallmark of cancer. MRSI provides the unique opportunity to collect metabolic fingerprints of tumor manifestations in vivo in patients with the ultimate goal of identifying markers for therapy stratification and monitoring.
 

Division of Radiopharmaceutical Development

German Cancer Consortium (DKTK), Partner Site Freiburg

Department of Nuclear Medicine

University Medical Center

Freiburg, Germany

Head of Biotechnological Development and Preclinical Imaging

Project Title: Development and translation of novel theranostic hybrid molecules for pre-, intra-, and postoperative diagnosis and therapy of cancer

Our research is focusing on identifying new biomarkers for the classification of tumors, assessing metastasizing potential and monitoring therapy. In particular my group is working on the development of small molecule peptides with a strong focus on hybrid molecules suitable for preoperative imaging, intraoperative navigation as well as for targeted endoradiotherapy of different cancer types. Therefore, we are using innovative biological, biotechnological and chemical approaches to identify novel peptides to a variety of clinically relevant target structures. Our development pipeline comprises the identification of potential binders, their detailed preclinical characterization including imaging studies finally leading to clinical translation.

Institute for Surgical Pathology

University Medical Center Freiburg

E-Mail: matthias.fahrner@uniklinik-freiburg.de

Project Title: In-depth proteogenomics of solid tumors to expand and complement routine molecular diagnostics and support personalized therapeutical strategies

Proteogenomics describes the integration of proteomics to multi-omics studies that integrate DNARNA-, and protein-level omics-data. In my research I apply in-depth proteogenomics on solid tumors
to address two different aspects:

a) Corroboration of determined sequence variants (DNA or RNA-level) on the proteome level; an approach which may also yield information on the proteomic penetrance of said variants. Results may aid in prioritization of potential treatment schemes if there is co-occurrence of multiple variants with clinical implications.

b) Determine the impact of genomic aberrations on oncogenic signaling pathways; many genomic aberrations are linked to oncogenic signaling and proteomic approaches enable corroborative, direct probing of this impact.

Institute of Surgical Pathology
University Medical Center Freiburg
E-Mail: melanie.foell@uniklinik-freiburg.de
Group Website

Project title: Spatially resolved metabolomics and proteomics of solid tumors
My junior research group “MALDI imaging” investigates tumor tissue cohorts and organotypic tissue slice cultures by MALDI mass spectrometry imaging and complementary omics and imaging modalities. MALDI imaging derives hundreds of molecular distribution maps directly from thin tissue slices. We apply MALDI imaging of metabolites and peptides to investigate spatially resolved tumor biology and to derive spatio-molecular signatures with diagnostic and prognostic potential. Furthermore, we establish open-source analysis tools and workflows in the Galaxy platform and work towards standardization and reproducibility of MALDI imaging, which lays the foundation for future clinical applications.
 

Department of Thoracic Surgery – Division of Cancer Research, University

Medical Center Freiburg
German Cancer Consortium (DKTK), Partner Site Freiburg
Breisacher Str. 115, 79106 Freiburg i.Br.

E-Mail: avantika.ghosh@dkfz-heidelberg.de

Project Titel: Systematic analysis of nonstop extension mutations in high throughput

Research in our group has shown that nonstop mutations are associated with cancer and are known to have a functional impact on protein expression and function. Our group has created the database NonStopDB, a comprehensive set of nonstop mutations in human cancer. In this project we aim to systematically analyse the functional impact of all non-stop extension mutations found somatically in human cancer in a pooled format using reporters and next generation sequencing. 

Department of Hematology, Oncology, and Stem Cell Transplantation

University Medical Center Freiburg

Project Title: Impact of CD30-mediated immune response in T-cell-lymphomas

The aim of this project is to get a better understanding of CD30-axis in disease progression in ALK-induced T-cell lymphomas with focus on immune response, immune escape mechanisms like immunoediting, involved pathways and other immune evasion mechanisms.

Universitätsklinikum Freiburg

Klinik für Innere Medizin I

Hämatologie, Onkologie und Stammzelltransplantation
Breisacherstr. 155 79106 Freiburg

E-Mail: eileen.haring@dkfz-heidelberg.de

Project Title: Genomic and transcriptomic analysis of leukemia cells and T cells 

Acute myeloid leukemia (AML) is the most common type of leukemia in adults and is characterized by a poor outcome. AML can be cured by an allogeneic hematopoietic cell transplantation (allo-HCT). During allo-HCT, the immune system of a donor is transferred into the patient and can eliminate residual malignant cells (graft-versus-leukemia effect, GVL).  However, immune escape and relapse are frequent in allo-HCT recipients.

In a currently ongoing clinical trial, we are studying the potential of immunotherapy with an anti-PD-1 antibody as a treatment for AML relapse after allo-HCT. This translational research project focusses on genomic, transcriptomic and phenotypic changes in leukemia and T cells of the patients undergoing immunotherapy. Peripheral blood and bone marrow mononuclear cells will be studied by sequencing technology and high-dimensional flow cytometry. Recognized alterations will be correlated with patient outcome and clinical parameters to identify predictive factors for the response to immunotherapy in allo-HCT recipients.

Institute of Molecular Medicine and Cell Research, Medical Faculty, Albert-Ludwigs-University Freiburg, D79104   Freiburg, Germany

Faculty of Biology, Albert-Ludwigs-University Freiburg, D79104 Freiburg, Germany

German Cancer Research Center (DKFZ), D69102 Heidelberg, Germany

German Cancer Consortium (DKTK), partner site Freiburg, D79106 Freiburg, Germany

Project Title: Degradome-wide genetic interference screening in murine breast cancer cells

In my PhD-project I am employing a degradome-wide inducible high-throughput genetic RNAi-based protease knockdown screes in two murine breast cancer cells lines to functionally analyze the role of proteases in breast cancer. Furthermore, I am investigating the functional connectivity between proteases and the highly cancer-associated PI3K-pathway using synthetic lethality screens.

Experimental Immunology

University Zürich

Switzerland

Project Title: The immune environment of acute myeloid leukemia

Acute myeloid leukemia (AML) is the most common hematologic malignancy in adults. It is characterized by poor outcome with an overall 5-year-survival of only 50% in young patients and 20% in elderly patients. As the curative potential of allogeneic hematopoietic stem cell transplantation is dependent on immunotherapeutic effects (graft-versus-leukemia effects), immune evasion seems to play a pivotal role in the pathogenesis of AML. A comprehensive research program using highdimensional single cell cytometry combined with bioinformatic analyses represents a powerful tool to gain deep and unbiased insights into the immune signatures of AML patients. Immune patterns that predict response may be integrated into the stratification of AML, particularly in the absence of predictive genetic markers. To verify the impact of the identified signatures, detailed functional analyses of patient mononuclear cells will be performed in vitro to investigate the effect of cell-intrinsic and -extrinsic alterations on anti-AML immunity. The ultimate goal is to identify novel stratifying biomarkers and/or therapeutic targets to overcome immune evasion of AML cells and achieve improved patient outcome.

Department of Radiology and Radiotherapy at the University Medical Center Freiburg

• German Cancer Consortium (DKTK), partner site Freiburg
• German Cancer Research Center (DKFZ)

Projects: 17O MRI for Regional CMRO2 Mapping; Development of Actively Visualized Devices and Safety of MRI-guided Interventions

MR Imaging with nuclei other than 1H provide additional information about tissue metabolism. MRI with the stable isotope 17O is used to quantify tissue oxygenation e.g. to optimize the treatment strategy in brain tumors. We develop dedicated imaging pulse sequences, novel reconstruction methods and radio frequency hardware to translate 17O MRI to clinical routine. 

MRI provides soft tissue contrast superior to x-ray technology and ultrasound. Therefore, MRI-guided operations such as biopsy, therapy, cardiovascular catheterization using active and passive devices such as metallic needles, metallic guidewires, electrodes, and active catheters becoming increasingly popular. We work on increasing the MRI visibility of interventional devices, assess the safety of those devices under MRI, and develop novel methods to investigate and eliminate potential safety risks.

Department of Thoracic Surgery – Division of Cancer Research, University Medical Center Freiburg

German Cancer Consortium (DKTK), Partner Site Freiburg

Breisacherstr. 115, 79106 Freiburg i.Br.

E-Mail: carla.schmidt@dkfz-heidelberg.de

Project Title: Discovering non-canonical driver mutations in cancer in high-throughput using endogenous models

Most point mutations found in cancer are functionally uncharacterized and hence cannot be exploited for patient stratification and therapy decisions. Current approaches to study mutations endogenously are time-consuming and lack the possibility for high-throughput. In this project, we focus on the introduction of comprehensive libraries of targeted mutations endogenously into the cell genome using innovative CRISPR-derived tools to assess their functional impact and their impact on therapy response and resistance.

Institut für Medizinische Bioinformatik und Systemmedizin

Medizinische Fakultät, Albert-Ludwigs-Universität Freiburg

Arbeitsgruppe Boerries für Systembiologie und Systemmedizin am DKFZ Partnerstandort Freiburg - DKTK

Institut für Molekulare Medizin und Zellforschung, Albert-Ludwigs-Universität Freiburg

Project Title: Genotypic and phenotypic characterization of patient-derived melanoma cell lines

Based on a transcriptome analysis, the aim of this project is to characterize the phenotypic properties of the four individual malignant melanoma cell lines, such as cell survival, proliferation, migration, and invasion. Furthermore, we are studying the underlying mechanisms, which effectuate the phenotype of the melanoma cell lines. Therein, we mainly focus on the MAPK/ERK or PI3K pathways, components of which are frequently mutated and thus are often constitutively active in cancer cells, driving their development and enhanced survival.

Project Title: Identification of early detection markers in pancreatic ductal adenocarcinoma (PDAC)

The aim of this project is to establish an early genetic classifier of PDAC based on a meta-analysis of independent studies. In combination with system biology and bioinformatics approaches, it is our aim to identify early markers of PDAC in patient-derived samples (eg. liquid biopsies). This may allow the differentiation of PDAC from early pancreatic intraepithelial neoplasia (PanIN), pancreatitis, and healthy tissues. In a parallel approach, we seek to inactivate the classifier genes in established PDAC cell lines and to assess the effects of the inactivation on the functionality of these cells.

Department of Radiation Oncology, University Hospital Heidelberg

Heidelberg Institute of Radiation Oncology (HIRO)

Heidelberg, Germany

E-Mail: Maximilian.deng@med.uni-heidelberg.de

Twitter: @maxiydeng1994

Project title: Molecularly-guided precision radiotherapy in patients with central nervous system tumors

The 2021 update of the WHO classification of central nervous system tumors has integrated comprehensive molecular findings into the diagnostic workflow, enabling a more accurate diagnostic process and risk assessment. Novel radiotherapeutic modalities using protons or carbon ions represent an auspicious therapeutic concept with an increased accuracy in dose delivery - while reducing the radiation dose for the surrounding organs at risk. Post-hoc integrated molecular-morphological scoring will identify patients at risk of progression, where proton- or carbon ion radiotherapy may be particularly beneficial, facilitating the path to a molecularly-guided treatment in patients with brain tumors.

Department of Medicine III

University Hospital RWTH Aachen

52074 Aachen, Germany

E-Mail: jkather@ukaachen.de

Medical Oncology, National Center for Tumor Diseases (NCT)

University Hospital Heidelberg

69120 Heidelberg, Germany

E-Mail: jakob.kather@nct-heidelberg.de

www.kather.ai 

Project Title: Computational Oncology

We are a young, interdisciplinary group of scientists using computational methods to decipher cancer. Our main tools are Deep Learning and Computational Modeling. We combine these tools with a clinical perspective on cancer genomics, targeted treatment and immunotherapy. Our focus is gastrointestinal cancer, including cancer of the bowel, stomach, liver and pancreas. To learn more about our research, have a look at our featured publications.

Department of Translational Medical Oncology

National Center for Tumor Diseases (NCT) Heidelberg

E-Mail: andreas.mock@nct-heidelberg.de

twitter: @am0ck

Project Title: RNA-centric Systems Biology for Precision Oncology

As a physician and data scientist within the NCT/DKTK MASTER (Molecularly Aided Stratification for Tumor Eradication Research) program, my research is concerned with developing RNA-based methodologies and predictive algorithms for Precision Oncology. Transcriptional signatures hold great promise to be the next generation predictive biomarkers as they enable the integration of oncogenic pathway activation states with the composition of the tumor immune microenvironment.

National Center for Tumor Diseases (NCT)

Department of Medical Oncology and German Cancer Research Center (DKFZ)

Signaling and Functional Genomics (Prof. Dr. Michael Boutros)

Heidelberg, Germany

E-Mail: a.schubert@dkfz.de

Twitter @_A_Schubert

Project Title: Novel strategies to analyze the role of Wnt-signaling in cancer

Deciphering the crosstalk of tumor cells and their surrounding stroma during cancer progression and metastasis is of highest importance to further optimize therapeutic strategies and prevent late recurrence. Wnt signaling governs cell faith and tissue polarity during development and aging. It was found dysregulated in various pathological processes and diseases such as cancer. This project aims to get further mechanistic insights into the complex Wnt-signaling networks using genome engineering and advanced imaging techniques that could lead to the identification of targets for earlier diagnostics and therapeutic intervention.

Department of Medical Oncology

National Center for Tumor Diseases (NCT) Heidelberg

Heidelberg, Germany

E-Mail: ​​​​​​t.walle@dkfz-heidelberg.de

Twitter: @science_wallet

Project Title: Systems immunodiagnostics platforms for combination cancer immunotherapy

The induction of effective anti-tumor immune responses is paramount for deep clinical responses to cancer immunotherapy. Here, we will develop a pan-tumor, pan-immunotherapy, low-cost, multiparametric and interpretable on-treatment biomarker for (combination) cancer immunotherapy. For this purpose, we will analyze changes in the serum proteome and peripheral blood mononuclear cell phenotype under steady state and controlled ex vivo perturbation using CITE-seq (10.1038/nmeth.4380), flow cytometry and mass spectrometry. Based on these omics data, we will construct a machine learning classifier to accelerate cancer immunotherapy development in adaptive clinical trials by a manifold.

Division of Stem Cells and Cancer (A010, Prof. Andreas Trumpp)

German Cancer Research Center (DKFZ)

Foundation under Public Law

Im Neuenheimer Feld 280

69120 Heidelberg

E-Mail: r.wuerth@dkfz.de 

https://www.dkfz.de/en/stammzellen-und-krebs/mitarbeiter.html

https://www.researchgate.net/profile/Roberto_Wuerth 

https://www.linkedin.com/in/wuerth/ 

ORCID: 0000-0002-8156-6756

Project Title: A liquid biopsies platform to study metastasis and to reveal individualized resistance mechanisms in breast cancer patient

Department of Gastroenterology, Technical University Hospital, Munich

E-Mail:  Melissa.Barroux@mri.tum.de

Project Title: Tumour evolution and immune microenvironment dynamics in response to neoadjuvant treatment in oesophagael adenocarcinoma

Patients with locally advanced oesophageal cancer (OAC) are treated with surgical resection and neoadjuvant chemotherapy or radiochemotherapy. However, 50% to 60% of tumours are resistant to neoadjuvant therapy, leading to an overall poor outcome with a 5-year survival of 12.6%. The ecological and evolutionary dynamics responsible for treatment failure are incompletely understood. In my research I perform a multi-omics study (whole exome sequencing, RNA-sequencing, T-cell receptor sequencing and image mass cytometry)  with a multi-timepoint strategy to examine treatment response at clonal resolution and to investigate genetic and transcriptomic changes induced by neoadjuvant therapy in OAC patients. This project will provide fundamental insights into clonal dynamics, transcriptomic changes and dynamics of the microenvironment in response to neoadjuvant treatment in OAC patients, in order to provide fundamental insights into treatment response on the molecular level.

Surgical resident at the Department of the Allgemein-, Viszeral- und Transplantationschirurgie

Ludwigs-Maximilians-University Munich

E-Mail: Mathilda.Knoblauch@med.uni-muenchen.de

Project Title: The effect of catecholamines and ß-ARs on the invasiveness of colorectal cancer cell

Tumor cell migration and invasion are multifactorially conditioned and the cause for metastasis development. Currently, it is poorly understood how and when cancer cells cross the endothelial barrier and proceed to metastasis. I focus on the aspect of the nervous system on several gastrointestinal and mesenchymal solid tumor entities in vitro and in vivo. My work deals with the influence of the nervous system in colorectal cancer metastasis and possible mechanisms in metastatic organotropism.

Klinikum rechts der Isar der Technischen Universität München
Institut für Klinische Chemie und Pathobiochemie

Center for Translational Cancer Research (TranslaTUM)

E-Mail: emma.morrish@tum.de

Project Title: Mechanism and regulation of aberrant T-cell receptor signalling in T-NHL

Recently, somatic gain-of-function mutations in T-cell receptor (TCR) signalling pathways have been described in T-cell non-Hodgkin lymphoma (T-NHL) patients, however their pathophysiological consequences remain ill-defined. Through generation of pre-clinical murine models, my research will study how these alterations affect immune signalling and lymphoma development to determine the molecular mechanisms driving disease. This project will provide fundamental insight into the pathomechanisms driving these aggressive T-cell cancers and lead to the identification of new negative regulators of TCR signalling.

Department of Otorhinolaryngology - Head and Neck Surgery & Walter Brendel Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-Universität München, Munich, Germany.

E-Mail: Bernd.Uhl@med.uni-muenchen.de

Project Title:  Characterization of myeloid leukocyte trafficking for novel therapeutic targets in cancer

The immune system protects the organism from infections, but also from the development of malignant tumors. Adaptive immune cells are well-known to control tumor initiation, progression, and metastasis formation in various cancer entities. During the last years, myeloid immune cells have additionally been recognized as essential players in these processes. The mechanisms underlying the trafficking of these immune cells to malignant lesions, however, remain largely unclear. Hence, the aim of our translational project is to characterize the mechanisms of myeloid leukocyte trafficking to malignant tumors to identify novel therapeutic targets for innovative immuno-oncological treatment strategies.

Kinderklinik München Schwabing

- Klinik und Poliklinik für Kinder- und Jugendmedizin, Klinikum Schwabing, München Klinik gGmbH und Klinikum Rechts der Isar (AöR) der Technischen Universität München -

Phone: 089/3068 2367
Email: hendrik.gassmann@tum.de

Project Title: Identification of tumor-antigen specific T cell receptors for the treatment of
pediatric cancers.

Despite significant survival improvements, cancer ranges among the leading causes of deaths in children and young adults. Immunotherapy including adoptive T cell transfer emerges as promising therapeutic strategy in pediatric hematological and solid malignancies. 
The short- and mid-term goals of the project are to identify and characterize T cell receptors (TCRs) in vitro and in preclinical models targeting overexpressed and shared antigens in pediatric cancers. The aim is to provide data for efficacy and safety of selected TCRs for further evaluation in phase I clinical trials.
 

Experimentelle Leukämie- und Lymphom-Forschung (ELLF), Medizinische Klinik und Poliklinik III, Klinikum der Universität München, Munich

Pathogenesis of acute leukemia - AG Prof. Dr. med. Philipp Greif 
Medizinische Klinik und Poliklinik III | Arbeitsgruppen (lmu-klinikum.de)

Email: Vanessa.Arfelli@med.uni-muenchen.de

Project Title: Alterations of fatty acid metabolism and pharmacological counteraction in ZBTB7A deficient leukemia

ZBTB7A is a transcription factor frequently mutated in acute myeloid leukemia (AML) patients with t(8;21) translocation (which results in the RUNX1-RUNX1T1 fusion protein). We use CRISPR-Cas9 to knockout ZBTB7A and mimic these loss-of-function mutations in order to study ZBTB7A effect in metabolism. Our ultimate goal is to find metabolic drug targets for therapy. For that, we perform metabolic tracing by mass spec, metabolic flux assays, and treatment with several metabolic inhibitors. Besides, since ZBTB7A prevents RUNX1-RUNX1T1-mediated clonal expansion in vitro, we are interested in investigating the cooperation of ZBTB7A knockout and RUNX1-RUNX1T1 during leukemogenesis in a mouse model.