DKTK Tech Talk: "From Protocol Search to Culture Success: Practical Insights into Establishing Patient-Derived Organoids" (Tim Schmäche)

Patient-derived organoids (PDOs) are increasingly used as physiologically relevant models for cancer research, offering unique opportunities for drug testing and personalized medicine. While the potential of PDOs is widely recognized, their successful establishment and long-term maintenance remain technically demanding and often require tumor-specific protocol adaptations.
In this TechTalk, Tim Schmäche will provide a practical overview of PDO generation from various gastrointestinal tumor entities. Topics will include tissue processing, protocol selection, medium optimization, and quality control strategies. I will also discuss common challenges and pitfalls encountered during culture establishment, and how to navigate them based on experience with a large PDO biobank. The goal is to share hands-on insights that may help others set up and refine their own PDO pipelines.

Registration